Spontaneous Acetylcholine Release Potentiation Induced by 2-Arachidonoylglycerol and Anandamide in Mouse Motor Synapses
| Autor: | A. E. Gaydukov, Olga P. Balezina, E. O. Tarasova, N. A. Khotkina |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Vesamicol 030302 biochemistry & molecular biology 2-Arachidonoylglycerol Long-term potentiation Anandamide Endocannabinoid system General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound chemistry Nitrendipine medicine Biophysics Inverse agonist lipids (amino acids peptides and proteins) General Agricultural and Biological Sciences Acetylcholine 030304 developmental biology General Environmental Science medicine.drug |
| Zdroj: | Moscow University Biological Sciences Bulletin. 76:1-6 |
| ISSN: | 1934-791X 0096-3925 |
| Popis: | Changes in the parameters of miniature endplate potential (MEPP) of mouse diaphragm caused by exogenous application of two classical endocannabinoids, 2-arachidonoylglycerol (2-AG) (1 μM) and anandamide (AEA) (30 μM), were compared. A slowly developing stable increase in the MEPP amplitude by 50%, without affecting the frequency of MEPPs, was caused by 2-AG. This effect was prevented by AM-251, an inverse agonist of CB1-receptors, as well as by vesamicol, a blocker of the vesicular acetylcholine (ACh) transporter. On the contrary, AEA did not cause significant changes in the MEPP amplitude but induced a slowly developing (within 2 h) increase in MEPP frequency by 75% on average. The effect of AEA was prevented by AM-251 (1 μM) as well as by blocking of L-type Ca2+-channels with nitrendipine (1 μM) and inhibition of PKA activity by H89 (1 μM). It was concluded that both 2-AG and AEA are able to exert a noncanonical facilitating presynaptic effect on spontaneous ACh release. Even though these endocannabinoids activate the same type of CB-receptors, their facilitating effects do not overlap and are strictly aimed either at potentiating the size of ACh quanta (in case of 2-AG) or increasing the frequency of MEPP (in case of AEA). We assume that different intracellular targets and signaling pathways may be involved in the differentiated facilitating effects of 2-AG and AEA in mouse neuromuscular junctions. |
| Databáze: | OpenAIRE |
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