Flavonoid profiling in three citrus varieties native to the Republic of Korea using liquid chromatography coupled with tandem mass spectrometry: contribution to overall antioxidant activity

Autor: Jae Hoon Kim, Gon-Sup Kim, A. M. Abd El-Aty, Sung Chul Shin, On Nuri Seo, Jong Sung Jin, Semin Park, Hae Gyeong Kim, Jung Han Lee, Soo-Jung Lee, Jae-Han Shim
Rok vydání: 2011
Předmět:
Zdroj: Biomedical Chromatography. 26:464-470
ISSN: 0269-3879
DOI: 10.1002/bmc.1688
Popis: A mixture of flavonoid components was isolated from the fruit peel of three varieties of citrus native to Republicof Korea, Citrus leiocarpa Hort. ex Tanaka (CLHT), Citrus aurantium L. (CAL) and Citrus erythrosa Hort. (CEH), via 70% methanolextraction followed by ethyl acetate elution over a silica gel cartridge. The flavonoid components of the mixture were ana-lyzed via high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) in positive-ion mode and acomparison ofthe reported data.Among 17 characterized components, twoflavanones, four flavones and twocoumarinderi-vatives in the fruit peel of the three varieties were identified for the first time. The individual characterized components werequantified via HPLC-UV. The flavanones dominated in CAL, whereas the flavones prevailed in CLHT and CEH. The antioxidantactivity of the flavonoid mixture of the fruit peel was determined via DPPH † , ABTS †+ and reducing power assays. The antiox-idant activity of CEH and CAL was greater than that of CLHT. Copyright © 2011 John Wiley & Sons, Ltd.Keywords: citrus; flavonoids; coumarin; HPLC-MS/MS; antioxidant activity
Databáze: OpenAIRE