Characterization of a cDNA Encoding a Novel Subunit for Cytochrome c Oxidase (COX6b) from Rice

Autor:	Atsushi Hirai, Mikio Nakazono, Katsumi Yamazaki, Kazuhiro Ohtsu, Sawako Hamanaka
Rok vydání:	1999
Předmět:	Oxidase test biology Cytochrome c Protein subunit Nucleic acid sequence food and beverages Plant Science Biochemistry Complementary DNA Genetics biology.protein Cytochrome c oxidase Agronomy and Crop Science Peptide sequence Southern blot
Zdroj:	Breeding Science. 49:211-215
ISSN:	1347-3735 1344-7610
DOI:	10.1270/jsbbs.49.211
Popis:	To date, there have been only a limited number of reports about the nuclear-encoded subunit for cytochrome c oxidase (COX) in the mitochondria of higher plants. In the present study, we characterized a cDNA encoding a novel subunit of COX (COX6b), which had not previously been reported in plants. It was found that the rice COX6b gene encodes 169 amino acids with a calculated molecular mass of 19 kDa. The deduced amino acid sequence of rice COX6b is longer than those of vertebrate and yeast COX6b subunits. Genomic Southern hybridization suggested that the rice COX6b subunit is encoded by at least two copies of COX6b genes. Northern hybridization showed that one of the genes (COX6b-1) is constitutively transcribed in different organs of rice. However, the steady-state mRNA levels slightly varied among organs.
Databáze:	OpenAIRE
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