C1 inhibitor deficiency enhances contact pathway mediated activation of coagulation and venous thrombosis

Autor: Steven Philip Grover, Tomohiro Kawano, Jun Wan, Pansakorn Tanratana, Zsofia Polai, Young Jun Shim, Omri Snir, Sigrid K. Brækkan, Sophia Dhrolia, Rohan Raj Kasthuri, Tem Bendapudi, Keith R McCrae, Alisa S. Wolberg, John-Bjarne Hansen, Henriette Farkas, Nigel Mackman
Rok vydání: 2023
Předmět:
Zdroj: Blood.
ISSN: 1528-0020
0006-4971
DOI: 10.1182/blood.2022018849
Popis: C1 inhibitor (C1INH) is a multifunctional serine protease inhibitor that functions as a major negative regulator of several biological pathways, including the contact pathway of blood coagulation. In humans, congenital C1INH deficiency results in a rare episodic bradykinin-mediated swelling disorder called hereditary angioedema (HAE). Patients with C1INH deficiency associated HAE (C1INH-HAE) have increased circulating markers of activation of coagulation. Further, we recently reported that patients with C1INH-HAE had a moderate but significant increased risk of venous thromboembolism. To further investigate the impact of C1INH deficiency on activation of coagulation and thrombosis, we conducted studies using patient samples and mouse models. Plasmas from patients with C1INH-HAE supported significantly increased contact pathway-mediated thrombin generation. C1INH-deficient mice, which have been used as a model of C1INH-HAE, had significantly increased baseline circulating levels of prothrombin fragment 1+2 and thrombin-antithrombin complexes. In addition, whole blood from C1INH-deficient mice supported significantly increased contact pathway-mediated thrombin generation. Importantly, C1INH-deficient mice exhibited significantly enhanced venous, but not arterial, thrombus formation. Further, purified human C1INH normalized contact pathway-mediated thrombin generation and venous thrombosis in C1INH-deficient mice. These findings suggest a key role for endogenous C1INH as a negative regulator of contact pathway-mediated coagulation in humans and mice. Further, this work identifies endogenous C1INH as an important negative regulator of venous thrombus formation in mice complementing the phenotype associated with C1INH-HAE.
Databáze: OpenAIRE
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