A Histochemical Technique for the Detection of Annonaceous Acetogenins
| Autor: | Alma Rosa González-Esquinca, Guillermo Laguna-Hernández, Iván De la Cruz-Chacón, Alicia Enriqueta Brechú-Franco |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
biology 010405 organic chemistry Stereochemistry food and beverages Tetrahydropyran 010402 general chemistry biology.organism_classification 01 natural sciences 0104 chemical sciences Endosperm chemistry.chemical_compound chemistry Annonaceae Reagent Annonaceous Acetogenins Tetrahydrofuran Lactone |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493967872 |
| DOI: | 10.1007/978-1-4939-6788-9_25 |
| Popis: | Annonaceous acetogenins (ACGs) are molecules with carbon numbers C35-C37, usually with tetrahydrofuran and tetrahydropyran rings and one terminal γ-lactone (usually α,β-unsaturated), in a large aliphatic chain that is varyingly hydroxylated, acetoxylated or ketonized. ACGs have ecological functions as insecticides and are pharmacologically promising due to their cytotoxic and antitumoral properties. They are found in the seeds, leaves, roots, flowers and fruits of annonaceous plants and can be detected during isolation via thin-layer chromatography using Kedde's reagent, which reacts with the unsaturated lactone. This chapter describes the location in situ of ACGs in fresh sections of annonaceous seeds using Kedde's reagent.The acetogenins are located in the idioblasts, in the endosperm and in the embryonic axis during differentiation. This method can aid in the detection of ACGs with a terminal unsaturated γ-lactone in organs and tissues. |
| Databáze: | OpenAIRE |
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