Effect of probiotics on patulin removal from synbiotic apple juice
| Autor: | Hossein Attar, Sayed Abolhasan Alavi, S Sohrabvandi, Kianoush Khosravi-Darani, Alaleh Zoghi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment 030106 microbiology law.invention Patulin 03 medical and health sciences Probiotic chemistry.chemical_compound 0404 agricultural biotechnology Lactobacillus acidophilus law medicine Food science Mycotoxin Nutrition and Dietetics biology Fructooligosaccharide Prebiotic 04 agricultural and veterinary sciences Ascorbic acid biology.organism_classification 040401 food science chemistry bacteria Agronomy and Crop Science Lactobacillus plantarum Food Science Biotechnology |
| Zdroj: | Journal of the Science of Food and Agriculture. 97:2601-2609 |
| ISSN: | 0022-5142 |
| DOI: | 10.1002/jsfa.8082 |
| Popis: | Studies have reported the occurrence of the mycotoxin patulin in apple products. The aim of this study was to produce synbiotic apple juice and investigate the detoxification of patulin by Lactobacillus acidophilus and Lactobacillus plantarum as probiotic strains. The impact of seven process variables on efficiency of toxin removal was investigated using Plackett-Burman design and presence of the surface-layer proteins as binding site of probiotics to patulin was confirmed during 6 weeks of cold storage.; Results: Results showed that the removal of patulin by probiotic bacteria from apple juice depends significantly (P < 0.05) on the fructooligosaccharide content (as a prebiotic), concentration of patulin and the addition of ascorbic acid. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of cell surface proteins of probiotic strains revealed that surface layer proteins have an important role in patulin removal from apple juice. In the best conditions, 91.23% of initial patulin concentration was removed from juice during 6 weeks refrigerated storage. No significant difference was observed in organoleptic properties of the synbiotic apple juice and raw sample.; Conclusion: In the best condition reported in this study, contaminated synbiotic apple juice by patulin will be safe for consumers after the first day of probiotic inoculation. © 2016 Society of Chemical Industry.; © 2016 Society of Chemical Industry. |
| Databáze: | OpenAIRE |
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