Endothelial vWf and t-PA release after incubation with different concentrations of asparaginase and dexamethasone
| Autor: | Johannes Wolff, Ulrike Nowak-Göttl, Joachim Boos, Gudrun Münstermann, M. Erben, B. Kehrel |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Asparaginase
medicine.medical_specialty biology Hematology Thrombomodulin chemistry.chemical_compound Endocrinology chemistry Von Willebrand factor Cell culture Plasminogen activator inhibitor-1 Internal medicine medicine biology.protein Plasminogen activator hormones hormone substitutes and hormone antagonists Dexamethasone Glucocorticoid medicine.drug |
| Zdroj: | Fibrinolysis. 10:55-56 |
| ISSN: | 0268-9499 |
| DOI: | 10.1016/s0268-9499(96)80049-x |
| Popis: | Summary Objective : to evaluate the possible influence of different concentrations of asparaginase (ASP), dexamethasone (Dex) or the combination ASP/Dex on porcine endothelial cells we measured von Willebrand factor (vWf), tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor 1 (PAI-1) and thrombomodulin (TM) in culture supernatant fractions. Results : vWf significantly increased in cells treated with 100 IU/ml ASP, but showed no difference in cells incubated with 1000 or 500 IU/ml ASP. t-PA concentrations were significantly increased in cells treated with 1000 and 100 IU/ml ASP. Dex alone and ASP/Dex resulted in a glucocorticoid dose-dependent increase of vWf and t-PA. PAI-1 and TM could not be determined in this cell line. Conclusion : these data provide evidence, that endothelial damage, occurring during treatment with ASP combined with Dex is mainly caused by glucocorticoid administration. |
| Databáze: | OpenAIRE |
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