Constructing a far-red to near-infrared fluorescent probe for highly specific detection of cysteine and its bioimaging applications in living cells and zebrafish
| Autor: | Hua Chen, Cai Fangyuan, Hong Liang, Xing-Can Shen, Shuping Zhang, Bo Hou, Cunji Gao |
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| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
biology Near-infrared spectroscopy Far-red 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Fluorescence Catalysis 0104 chemical sciences Amino acid chemistry Materials Chemistry Thiol Biophysics 0210 nano-technology Zebrafish Intracellular Cysteine |
| Zdroj: | New Journal of Chemistry. 43:6696-6701 |
| ISSN: | 1369-9261 1144-0546 |
| DOI: | 10.1039/c9nj00260j |
| Popis: | Cys is a common and important sulfur-containing amino acid in living organisms, whose intracellular level changes are associated with a variety of diseases. To specifically detect Cys without interference from other thiol species has considerable significance. However, only selective fluorescent probes are available for cysteine detection; other thiol species can also have certain interference in the sensing process. Herein, we report an irreversible fluorescent probe for the specific detection of cysteine without interference from other thiol species both in living cells and zebrafish. The FR-NIR-Cys probe exhibits a large emission wavelength shift (up to 84 nm) and a significant ratio fluorescence enhancement (up to 41-fold) in the far-red and near-infrared region. Furthermore, we demonstrate that FR-NIR-Cys with good cell permeability can be employed in the far-red to near-infrared imaging of Cys in living cells and zebrafish. |
| Databáze: | OpenAIRE |
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