Abstract 277: Nox Isoforms, Oxidative Stress, Inflammation And Fibrosis In Adipose Tissue In Obese Mice: Differential Regulation In Epididymal, Perirenal And Perivascular Depots
| Autor: | Sarah E Even, Augusto C Montezano, Francisco J Rios, Aurélie Nguyen Dinh Cat, Andrew Carswell, Ying He, Tayze T Antunes, Glaucia E Callera, Karin Jandeleit-Dahm, Mark Cooper, Cedric Szyndralewiez, Patrick Page, Rhian M Touyz |
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| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Hypertension. 64 |
| ISSN: | 1524-4563 0194-911X |
| Popis: | Adipose tissue inflammation and fibrosis contribute to cardiovascular complications observed in obese patients. Mechanisms related to these deleterious effects are still elusive. Here, we evaluated the oxidative, fibrotic and inflammatory status of obese mice and the putative role of NADPH oxidases. Epididymal (EP), perivascular (PV) and peri-renal (PR) fat were collected from lean (db/m) and obese (db/db) mice. Gene expression was assessed by qPCR, fibrosis by picro Sirius red staining and polarized light microscopy, oxidative stress by Amplex Red (H2O2) and TBARS. In EP fat, white adipose tissue, increased levels of TBARS (56%), CD206 mRNA (macrophage marker - 106%) and fibrosis (300%) were observed in obese mice (p |
| Databáze: | OpenAIRE |
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