| Popis: |
OBJECTIVE Pre-analytical phase in laboratory testing is the serious part of analytical process which has impact on laboratory results. In these regard time delays between collection of samples and analysis are unavoidably common and samples are keep stored in refrigerator which might have effect on the accuracy of results. RESULTS Serum samples were obtained from 20 apparently healthy horse and cattle and aliquots of serum were divided in to three tubes. Two tubes (T1 and T2) were stored at -20°C for one and two months respectively while the other used for base line (T0) analysis. Total cholesterol, Triglycerides, Total protein, albumin and Urea were the tested analytes. Analysis of variance for selected analytes shown that there were no significant (P>0.05) differences in the mean Total Cholesterol, Total Protein, Albumin, Urea and Triglycerides values in T1 and T2 post storage, when compared to the baseline T0. The study also examined the changes in terms of total allowable error TEa (%) adopted from the American society of veterinary clinical pathology (ASVCP) guidelines and found that total observed error TEo (%) for the specified analytes was within the TEo(%). The results indicated that all analyses were stable up to 2 months when stored at -20°C; however a stability studies at different storage temperature, time intervals, and laboratory methods is recommended to fully validate their stability. |
