DETECÇÃO DE BETALACTAMASES DE ESPECTRO ESTENDIDO (ESBL) EM ISOLADOS DE Escherichia coli UROPATOGÊNICAS (UPECs) ORIUNDOS DE PACIENTES DA COMUNIDADE
| Autor: | Adriana de Mendonça Marques, Cristina Monteiro, Valério Monteiro-Neto, Patrícia de Maria Silva Figueiredo, Maria Rosa Quaresma Bomfim, Luis Henrique Bastos Gonçalves, Rosimary de Jesus Gomes Turri, Thiago Azevedo Feitosa Ferro, Monique Santos do Carmo |
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| Rok vydání: | 2012 |
| Předmět: |
General Immunology and Microbiology
Gram-negative bacterial infections medicine.drug_class Antibiotics Public Health Environmental and Occupational Health Biology bacterial infections and mycoses Antimicrobial biology.organism_classification medicine.disease_cause Microbiology Infectious Diseases Multiplex polymerase chain reaction medicine Gene Escherichia coli Bacteria |
| Zdroj: | Revista de Patologia Tropical. 41 |
| ISSN: | 1980-8178 0301-0406 |
| Popis: | The production of ESBL is an important mechanism of resistance to ?-lactam antimicrobials. The wide use of antibiotics in the treatment of urinary tract infections (UTI) has further aggravated this problem. Bacteria of the UPEC type are the main responsible for this kind of infection. Studies to understand the diversity of ESBL in Brazil are scarce and, in addition, many of them are directed to nosocomial samples. The objective of this study was to detect genes encoding ?-lactamases TEM, SHV and CTX-M through multiplex PCR, as well as to identify the isolates that produce them through the disk approach method. From the total of isolates analyzed, 12.8% showed a positive phenotype for ESBL, while 65.8% amplified sequences homologous to the genes of ?-lactamases. Among these, the most prevalent were blaTEM and blaCTX-M, with 42.7% and 18.8%, respectively. Two isolates did not amplify for any genes in this study, despite of having expressed the enzyme in the phenotypic test. Based on to our results, we suggest the development of further studies aiming at the molecular characterization to better elucidate the mechanism of spread of resistance among bacteria from patients in the community. |
| Databáze: | OpenAIRE |
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