Estimation of flavonoid and centelloside accumulation in leaves of Centella asiatica L. Urban by multiparametric fluorescence measurements
| Autor: | Michaela Schmitz-Eiberger, Mauricio Hunsche, Viola Müller, Christa Lankes, Georg Noga |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Centella biology Phosphorus fungi Flavonoid Saponin food and beverages chemistry.chemical_element Plant Science biology.organism_classification chemistry.chemical_compound Flavonols chemistry Anthocyanin Botany Food science Medicinal plants Secondary metabolism Agronomy and Crop Science Ecology Evolution Behavior and Systematics |
| Zdroj: | Environmental and Experimental Botany. 93:27-34 |
| ISSN: | 0098-8472 |
| DOI: | 10.1016/j.envexpbot.2013.05.001 |
| Popis: | a b s t r a c t Recently, we have shown the relevance of nitrogen (N), phosphorus (P) and potassium (K) supply levels for resource partitioning between primary and secondary metabolism, and the concentration of centel- losides in Centella asiatica L. Urban leaves. So far, no efforts have been made to investigate the effects of mineral supply on flavonoid concentrations in this species. Here, we aimed to examine the accumula- tion of centellosides in C. asiatica leaves in vivo by means of non-destructive fluorescence measurements using products of the secondary metabolism, particularly the epidermal flavonols and anthocyanins, as reference. For this purpose we conducted three discrete experiments in a greenhouse having N, P and K levels as experimental factors. Our results reveal that flavonoid and anthocyanin accumulation is affected by N, P and K fertigation in the same way as the centelloside accumulation. More precisely, limitations in plant growth were accompanied by higher flavonoid and anthocyanin concentrations, confirming the pro- posed trade-off between the plant's primary and secondary metabolism. The fluorescence-based flavonol (FLAV) and anthocyanin (ANTH RG) indices correlated fairly with flavonoid and especially with antho- cyanin concentrations. Moreover, centellosides were positively correlated with the FLAV and ANTH RG indices, and with the BFRR UV index, which is considered as universal 'stress-indicator'. Thus, here we indicate for the first time, that the fluorescence-based indices FLAV, ANTH RG as well as BFRR UV enable the monitoring of flavonoid and centelloside concentrations in leaves of C. asiatica. Our results support and highlight the significant potential for further development and application of fluorescence-based sensors in ecophysiological research as well as in the production of medicinal plants. |
| Databáze: | OpenAIRE |
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