EVIDENCE OF A LATENT OXIDATIVE BURST IN RELATION TO WOUND REPAIR IN THE GIANT UNICELLULAR CHLOROPHYTE DASYCLADUS VERMICULARIS1

Autor:	Frithjof C. Küpper, Valerie Vreeland, J. Herbert Waite, Clifford Ross, Robert S. Jacobs
Rok vydání:	2005
Předmět:	Wound site integumentary system biology Kinetics Plant Science Oxidative phosphorylation Dasycladus Aquatic Science biology.organism_classification Respiratory burst chemistry.chemical_compound chemistry Biochemistry biology.protein Biophysics Dasycladus vermicularis Hydrogen peroxide Peroxidase
Zdroj:	Journal of Phycology. 41:531-541
ISSN:	1529-8817 0022-3646
DOI:	10.1111/j.1529-8817.2005.04072.x
Popis:	We investigated the kinetics and composition of the second phase of the wound repair process of Dasycladus vermicularis ([Scropoli] Krasser) using fluorescent probes, chromatography, UV spectroscopy, and histochemistry. Our new evidence supports the hypothesis that the second phase of wound repair (initiated at approximately 35-45 min post-injury) is based on the activation of an oxidative burst that produces micromolar H 2 O 2 levels. These results provide evidence of peroxidase activity at the wound site, real-time measurements of an oxidative burst, and catechol localization in wound plugs. Strong evidence is presented indicating that the biochemical machinery exists for oxidative cross-linking to ensue in the wound-healing process of D. vermicularis.
Databáze:	OpenAIRE
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