| Autor: |
Miroslaw Tarnawski, Lin Xue, Jochen Reinstein, Nicole Mertes, Jochen Wilhelm, J. Tuennermann, J. Karpenko, T. Taenzer, S. Kuehn, G. Gotthard, Julien Hiblot, Kai Johnsson, U. Uhrig |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.04.13.439540 |
| Popis: |
The self-labeling protein tags (SLPs) HaloTag7, SNAP-tag and CLIP-tag allow the covalent labeling of fusion proteins with synthetic molecules for applications in bioimaging and biotechnology. To guide the selection of an SLP-substrate pair and provide guidelines for the design of substrates, we report a systematic and comparative study on the labeling kinetics and substrate specificities of HaloTag7, SNAP-tag and CLIP-tag. HaloTag7 reaches almost diffusion-limited labeling rates with certain rhodamine substrates, which are more than two orders of magnitude higher than those of SNAP-tag for the corresponding substrates. SNAP-tag labeling rates however are less affected by the structure of the label than those of HaloTag7, which vary over six orders of magnitude for commonly employed substrates. Solving the crystal structures of HaloTag7 and SNAP-tag labeled with fluorescent substrates allowed us to rationalize their substrate preferences. We also demonstrate how these insights can be exploited to design substrates with improved labeling kinetics. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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