Effects of 17β-Estradiol on the Incorporation of Pyrimidine Nucleotide Precursors into Nucleotide Pools and RNA in the Mouse Uterus1
| Autor: | Billy Baggett, Bevan G. Miller |
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| Rok vydání: | 1972 |
| Předmět: | |
| Zdroj: | Endocrinology. 90:645-656 |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | The effects of 17β-estradiol on the incorporation of several pyrimidine nucleotide precursors into uterine RNA and free nucleotide pools have been investigated. Total RNA commenced increasing by 6 hr after administration of 0.05 μg 17β-estradiol, and was 60% above the control level at 10 hr. The size of the total free nucleotide pool did not change during the first 6 hr, then increased to 20% above the control level at 10 hr. In mice receiving [5-3H] cytidine or [2-14C] uridine iv 20 min before killing, the specific activities (SA) of both RNA and total free nucleotides increased to maximum values at 4 hr after 17β-estradiol administration of about ten times the respective control levels. However, in mice receiving iv injections of [5-3H] orotic acid, the SA of the total free nucleotides increased only slowly after giving 17β-estradiol, to 47% above the control level at 4 hr. The SA of RNA in the same mice increased promptly to a maximum at 2 hr of almost twice the control level. Estrogen stimulation incr... |
| Databáze: | OpenAIRE |
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