Abstract TMP31: Exosomes Role in Brain Repair and Recovery in Stroke. A Dose Response Study
| Autor: | Laura Otero-Ortega, Luke Diekhorst, Mercedes Arrúe Gonzalo, Fernando Laso-García, Mari Carmen Gómez-de Frutos, Blanca Fuentes, María Gutiérrez-Fernández, Arturo Martínez Arroyo, Exuperio Díez Tejedor, Elisa Alonso López |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Advanced and Specialized Nursing Minimal effective dose business.industry medicine.medical_treatment Mesenchymal stem cell Adipose tissue Stem-cell therapy medicine.disease Microvesicles Dose Response Study Brain repair 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Cancer research medicine Neurology (clinical) Cardiology and Cardiovascular Medicine business Stroke 030217 neurology & neurosurgery |
| Zdroj: | Stroke. 49 |
| ISSN: | 1524-4628 0039-2499 |
| Popis: | Aim: To assess the efficacy of 3 doses of exosomes from adipose tissue derived human mesenchymal stem cells to identify the minimal effective dose to enhance brain repair and recovery after subcortical stroke in rats. Methods: Male and female Sprague-Dawley rats (250gr) were injected with endothelin-1 to induce subcortical stroke. 24h after stroke, exosomes were administrated by the tail vein. Study groups were: Sham (n=10): without stroke neither treatment; control: stroke+saline (n=8); stroke+low dose (50μg) (n=10); stroke+intermediate dose (100μg) (n=9); stroke+high dose (200μg) (n=10). We evaluated: motor function (Roger’s, Rotarod and Walking beam test) at 24h, 7 and 28d, lesion volume and tract connectivity were studied by magnetic resonance image at 24h and 28d, brain repair markers: GFAP (Glial Fibrillary Acidic Protein), MOG (Myelin Oligodendrocyte Glycoprotein), MBP (Myelin Basic Protein) by immunofluorescence at 28d. Results: Animals treated with 50μg, 100μg or 200μg of exosomes showed a significant improvement in functional evaluation compared to control (p Conclusion: Intravenous administration of different doses of exosomes (50μg, 100μg and 200μg) improves the functional recovery, tract connectivity and expression of brain repair markers. Low dose is as effective as intermediate and high doses. Therefore, 50μg of exosomes is the minimal effective dose to enhance brain repair and recovery in stroke.From a translational point of view, this dose decreases the cost and risk. |
| Databáze: | OpenAIRE |
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