Memory immune mechanisms against parasitic tissue-dwelling Heligmosomoides polygyrus larvae (133.25)
| Autor: | Timothy Kreider, Andrew G Gaydo, Joseph F Urban, Jr., William C Gause |
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| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 182:133.25-133.25 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.182.supp.133.25 |
| Popis: | The nematode parasite H. polygyrus has both a parasitic intestinal tissue-dwelling larval phase and a lumen-dwelling adult phase in its life cycle. Primary inoculation of naïve mice resulted in chronic infection while secondary inoculation after drug-cure to clear adult worms triggered a protective Th2-type memory response, characterized by a distinct Th2-type granuloma that developed by 4 days after secondary but not primary inoculation. We investigated the effects of this memory response on early larval development. As early as day 4 after inoculation, parasitic larvae in the intestine were fewer in number, exhibited shorter lengths, and were more widely distributed across the small intestine in the memory compared to the primary response. Mice administered DMFO, which inhibits ornithine decarboxylase, after secondary inoculation exhibited significantly increased larval numbers and lengths, but no change in parasite distribution, suggesting an important role for polyamines in sustaining a host-protective memory response. In addition, B cell-deficient mice contained larvae with increased lengths and altered distribution in the intestine after secondary inoculation that was comparable to the response observed after a primary inoculation. The early host-protective response appeared to be TSLP-independent because it was not impaired in TSLPR-/- mice. These studies suggested that memory Th2-type responses impaired tissue-dwelling parasite migration and development as early as four days after infection, and that this response was partly dependent on polyamine production and B cells. |
| Databáze: | OpenAIRE |
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