Change in Hatching Enzyme Activity during Development of the Sea Urchin, Hemicentrotus Pulcherrimus

Autor:	Mitsunobu Nakatsuka
Rok vydání:	1981
Předmět:	chemistry.chemical_classification animal structures biology Hatching Ecology Embryo Cell Biology biology.organism_classification Enzyme assay Hemicentrotus Enzyme chemistry Biochemistry biology.animal embryonic structures biology.protein Secretion Cell fractionation Sea urchin Developmental Biology
Zdroj:	Development, Growth and Differentiation. 23:371-380
ISSN:	1440-169X 0012-1592
DOI:	10.1111/j.1440-169x.1981.00371.x
Popis:	The time course of change in hatching enzyme activity during development of embryos of the sea urchin Hemicentrotus pulcherrimus was observed. The enzyme was present in the particulate fraction in embryos until the time of hatching and was maximal at the time of hatching. Cell fractionation studies suggested the existence of an inhibitor of the hatching enzyme. This possibility was subsequently substantiated by experiments in mixtures of fractions: the activity of hatching enzyme in the particulate fraction was inhibited by the supernatant of embryos. This inhibitory factor was heat-stable and non-dialyzable, but it was not characterized further. The activity of secreted hatching enzyme was not inhibited by this factor, suggesting that the molecular forms of hatching enzyme in embryos and in the culture supernatant are different. After hatching, the amount of increase in the hatching enzyme activity in the culture supernatant was 3.5 times the amount of decrease in enzyme activity in the embryos, suggesting that the enzyme was activated during its secretion.
Databáze:	OpenAIRE
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