Interleukin-4 enzyme-linked immunospot assay may be useful for diagnosing sensitization to house dust mite

Autor:	Yeon-Hee Joo, Hyunmo Kang, Rock Bum Kim, Sea-Yuong Jeon, Jino Lee, Hyun-Jin Cho, Sung-Won Choi, Sang-Wook Kim, Dong-Yeop Chang, Hyeong Joo Lee, Seong Chul Yeo
Rok vydání:	2016
Předmět:	0301 basic medicine House dust mite Allergy biology Receiver operating characteristic business.industry ELISPOT biology.organism_classification medicine.disease medicine.disease_cause Immunoglobulin E 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Allergen 030228 respiratory system Otorhinolaryngology Immunology medicine biology.protein Immunology and Allergy business Sensitization Interleukin 4
Zdroj:	International Forum of Allergy & Rhinology. 6:1007-1012
ISSN:	2042-6976
DOI:	10.1002/alr.21786
Popis:	Background The skin prick test (SPT) is considered a standard test for identification of allergens, but it has some limitations in clinical practice. The multiple allergen simultaneous test (MAST), which measures allergen-specific immunoglobulin E in patients’ serum, is a widely used alternative test, but is limited by its relatively low sensitivity and specificity. As a novel diagnostic test to identify allergens, we investigated the sensitivity and specificity of an interleukin-4 (IL-4) enzyme-linked immunospot (ELISpot) assay for Dermatophagoides farinae (Der f) and Dermatophagoides pteronyssinus (Der p). Methods Based on the symptoms and SPT results, 43 house dust mite (HDM) allergic rhinitis (AR) patients and 41 control subjects were included. Peripheral blood was drawn from each subject for IL-4 ELISpot assay and MAST. The receiver operating characteristic (ROC) curve analysis was conducted to determine the cutoff values. Sensitivity, specificity, and positive and neg predictive values were compared between the 2 tests. Results The sensitivity, specificity, and areas under the ROC curve (AUCs) of the IL-4 ELISpot assay were 88.4%, 97.6%, and 0.939 for Der f, and 95.3%, 97.5%, and 0.971 for Der p, respectively. However, the sensitivity, specificity, and AUC of MAST were 76.7%, 73.2%, and 0.777 for Der f, and 69.8%, 75.6%, and 0.788 for Der p, respectively. Conclusion The IL-4 ELISpot assay showed higher sensitivity, specificity, and AUC than MAST, which indicates its clinical feasibility for diagnosing allergy for HDM. A further study is needed to determine the accuracy of the IL-4 ELISpot assay for other common allergens.
Databáze:	OpenAIRE
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