Mixed surfactant modified graphene oxide nanocarriers for DOX delivery to cisplatin-resistant human ovarian carcinoma cells
| Autor: | Mingzhi Tang, Jiabin Chen, Bing Liu, Guolin Li, Yushi Liu, Huirong Chi, Qian Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
Chromatography
endocrine system diseases General Chemical Engineering technology industry and agriculture macromolecular substances 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Controlled release 0104 chemical sciences carbohydrates (lipids) chemistry.chemical_compound Pulmonary surfactant chemistry Drug delivery polycyclic compounds Biophysics Zeta potential Doxorubicin Hydrochloride Nanocarriers 0210 nano-technology Cytotoxicity Hydroxyethyl cellulose |
| Zdroj: | RSC Advances. 6:87258-87269 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c6ra17609g |
| Popis: | The non-covalent functionalization of graphene oxide (GO) with hydroxyethyl cellulose (HEC, nonionic surfactant) and polyanionic cellulose (PAC, anionic surfactant) was developed for anti-cancer drug delivery applications based on a nonideal mixed micelle theory of surfactants. In order to maintain the stability of GO in aqueous electrolytes, the optimal component of the mixed surfactant modified nanocarriers (GO–PAC/HEC) was determined via a multi-factors orthogonal method. Benefiting from the enormous specific surface area of GO and π–π stacking interactions, doxorubicin hydrochloride (DOX), a model anticancer drug, was loaded onto the GO–PAC/HEC surface with high drug loading. The most appropriate loading content of DOX on GO–PAC/HEC was obtained by zeta potential evaluation and a stability time test. The in vitro DOX release profiles from DOX-loaded nanocarriers (GO–PAC/HEC–DOX) exhibited a significant pH-responsive feature and satisfactory performance of drug controlled release. Moreover, in vitro investigation showed that DOX-loaded nanoparticles entered into and treated cancer cells effectively and enhanced the accumulation of DOX in SKOV3/DDP cells (cisplatin-resistant human ovarian carcinoma cell line) exhibiting higher cytotoxicity than free DOX, suggesting that DOX-loaded nanoparticles may overcome DOX resistance of SKOV3/DDP cells to some extent. Based on the results obtained in this study, GO–PAC/HEC shows considerable potential as a drug delivery vehicle. |
| Databáze: | OpenAIRE |
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