Visualizing Ebolavirus Particles Using Single-Particle Interferometric Reflectance Imaging Sensor (SP-IRIS)
| Autor: | Steven M. Scherr, John H. Connor, Erik Carter, David S. Freedman, Elif Seymour, M. Selim Ünlü, George G. Daaboul |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Ebolavirus Ebola virus Materials science business.industry viruses 02 engineering and technology 021001 nanoscience & nanotechnology medicine.disease_cause law.invention 03 medical and health sciences Interferometry 030104 developmental biology Optics law medicine Particle IRIS (biosensor) Electron microscope Image sensor 0210 nano-technology business Biosensor |
| Zdroj: | Ebolaviruses ISBN: 9781493971152 |
| Popis: | This chapter describes an approach for the label-free imaging and quantification of intact Ebola virus (EBOV) and EBOV viruslike particles (VLPs) using a light microscopy technique. In this technique, individual virus particles are captured onto a silicon chip that has been printed with spots of virus-specific capture antibodies. These captured virions are then detected using an optical approach called interference reflectance imaging. This approach allows for the detection of each virus particle that is captured on an antibody spot and can resolve the filamentous structure of EBOV VLPs without the need for electron microscopy. Capture of VLPs and virions can be done from a variety of sample types ranging from tissue culture medium to blood. The technique also allows automated quantitative analysis of the number of virions captured. This can be used to identify the virus concentration in an unknown sample. In addition, this technique offers the opportunity to easily image virions captured from native solutions without the need for additional labeling approaches while offering a means of assessing the range of particle sizes and morphologies in a quantitative manner. |
| Databáze: | OpenAIRE |
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