A study of the origin of human glioma based on cell lines and tumor specimens
| Autor: | E. N. Tolkunova, Yu. S. Lakhina, D. A. Gulyaev, G. V. Timin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Human glioma Vimentin Cell Biology Biology medicine.disease Tumor tissue OLIG2 03 medical and health sciences 030104 developmental biology 0302 clinical medicine nervous system Cell culture Cancer stem cell 030220 oncology & carcinogenesis Glioma Immunology biology.protein Cancer research medicine NeuN |
| Zdroj: | Cell and Tissue Biology. 10:100-105 |
| ISSN: | 1990-5203 1990-519X |
| Popis: | This study is focused on the search for human glioma “cells of origin.” Specimens of tumor tissue have been assayed with RT-PCR for the expression of molecular markers specific for nerve tissue (NeuN, MOG, MBP, NG2, Olig2, Vimentin, GFAP, Aldh1L1), as well as markers of stem (Oct4, C-Kit) and cancerous stem (CD133) cells. It was found that the expression profiles of these markers were overlapped for different types of gliomas and the type of “cells of origin” cannot be determined. We suggest that more sophisticated culture conditions than traditionally used serum-based media should be applied to study the origin of glioma based on cell lines. |
| Databáze: | OpenAIRE |
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