Pathogenicity and Virulence of Purpureocillium lilacinum (Hypocreales: Ophiocordycipitaceae) on Mexican Fruit Fly Adults
| Autor: | Jorge Toledo, Pablo Liedo, Graciela Huerta-Palacios, Ricardo Alberto Toledo-Hernández, Javier Valle-Mora, Francisco Holguín-Meléndez |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Ophiocordycipitaceae biology Hypocreales Biological pest control Fecundity biology.organism_classification 010603 evolutionary biology 01 natural sciences Conidium 010602 entomology Horticulture Purpureocillium lilacinum Insect Science Tephritidae Anastrepha ludens Ecology Evolution Behavior and Systematics |
| Zdroj: | Florida Entomologist. 102:309 |
| ISSN: | 0015-4040 |
| DOI: | 10.1653/024.102.0204 |
| Popis: | Purpureocillium lilacinum (Thom) Luangsa-ard, Houbraken, Hywel-Jones & Samson (Hypocreales: Ophiocordycipitaceae) is a fungus commonly used for controlling nematodes, and also has been reported as an insect pathogen. However, little is known about its effects on insects. Here, the pathogenicity of 9 isolates and the virulence and sublethal effects of 2 isolates were evaluated to control adult Anastrepha ludens (Loew) (Diptera: Tephritidae). The pathogenicity assays demonstrated that the 9 isolates were pathogenic, with mortality percentages ranging from 28.8 to 52.4% and LT50 values were 18 d or more. The strain CFFSUR-A53 was more virulent than CFFSUR-A60, with LC50 values of 7.62 × 106 and 5.2 × 109 conidia per mL, respectively. The isolates reduced the life expectancy of the flies by 65 and 37%, decreased fecundity by 78 and 36%, and reduced egg hatching by 31.4 and 18.5%, respectively. Resumen Purpureocillium lilacinum (Thom) Luangsa-ard, Houbraken, Hywel-Jones & Samson (Hypocreales: Ophiocordycipitaceae) es un hongo comunmente utilizado en el control de nematodos y ha sido reportado como patogeno de insectos. Sin embargo, son poco conocidos los efectos que causa sobre los insectos. Aqui se evaluo la patogenicidad de nueve aislamientos y la virulencia y efectos subletales de dos aislamientos en adultos de Anastrepha ludens (Loew) (Diptera: Tephritidae). Los ensayos demostraron que los nueve aislamientos fueron patogenicos, y causaron porcentajes de mortalidad que oscilaron entre 28.8 y 52.4% y un TL50 de 18 d o mas. La cepa CFFSUR-A53 fue mas virulenta que la cepa CFFSUR-A60, con valores de CL50 de 7.62 × 106 y 5.2 × 109 conidios por mL, respectivamente. Ambos aislamientos redujeron en 65 y 37% la esperanza de vida de las moscas, disminuyeron en 78 y 36% la fecundidad de las hembras, y en 31.4 y 18.5% la eclosion de los huevos, respectivamente. Key Words: entomopathogenic fungi; biological control; Anastrepha ludens, fecundity and fertility View this article in BioOne https://doi.org/10.1653/024.102.0204 |
| Databáze: | OpenAIRE |
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