Under flow impedimetric measurements using magnetic particles for label-free detection affinity target

Autor:	Walid Mohamed Hassen, Adnane Abdelghani, Nicole Jaffrezic-Renault, Lúcio Angnes, François Bessueille, Didier Léonard
Rok vydání:	2008
Předmět:	Streptavidin Materials science Atomic force microscopy Bioengineering Nanotechnology Signal Buffer (optical fiber) Dielectric spectroscopy Biomaterials chemistry.chemical_compound chemistry Flow (mathematics) Mechanics of Materials Magnetic nanoparticles Fourier transform infrared spectroscopy
Zdroj:	Materials Science and Engineering: C. 28:820-825
ISSN:	0928-4931
DOI:	10.1016/j.msec.2007.10.041
Popis:	The necessity to adapt sensors based on electrochemical techniques for high throughput analysis control increases the interest to develop new analytical systems able to perform measurements under buffer flow. In this report we explored the possibility of employing a new system to make impedimetric measurements to detect the interaction between proteins and small molecules. The well-known biotin–streptavidin interaction was adopted to evaluate the proposed assembly. This system allows us to perform experiments under flow. Magnetic beads functionalized with streptavidin were used and first characterized using AFM and FTIR. Non-faradic impedance spectroscopy allowed the detection of the biotin–streptavidin interaction. Using our new system and under a flow of PBS buffer, 5 10− 5 M of biotin was detected with a stable signal.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f782397ed2383fb44d28c8b4f9404b0b https://doi.org/10.1016/j.msec.2007.10.041 Zobrazit plný text záznamu Full Text from ScienceDirect