| Popis: |
Stingrays envenomation in humanswere the common accident in the marine and freshwater ecosystem. To determine such effect species Himantura imbricata have been used to elucidate Hemolytic activity, Plasma Coagulation, Fibrin coagulation, Fibrinolytic activity, Activated Partial Thromboplastin Time (APTT),and Prothrombin Time (PT) effects were studied. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Amino acid analysis by RP-HPLC, FTIR spectral analysis and SEM were carried for characterization studies. The results show the of plasma coagulation, fibrin coagulation, of this stingray venom delays the coagulation of citrated plasma. APTT and PT results showed intrinsic and extrinsic coagulation factors that were responsible in time delay when compared with the control. Moreover, these biological experiments and characterization studies aided in understanding the envenoming factors and these results might base the development of treatments for complex diseases. |
