Differential protein profiles ofBradyrhizobium japonicumUSDA110 bacteroid during soybean nodule development

Autor: Hatthaya Arunothayanan, Satoshi Tabata, Takakazu Kaneko, Tan Van Dao, Shusei Sato, Hoa Thi Phuong Le, Mika Nomura, Shigeyuki Tajima
Rok vydání: 2010
Předmět:
Zdroj: Soil Science and Plant Nutrition. 56:579-590
ISSN: 1747-0765
0038-0768
DOI: 10.1111/j.1747-0765.2010.00500.x
Popis: During nodule development of legumes, together with the morphological alteration of plant cells, the rhizobia undergo marked biochemical and physiological changes and differentiate to bacteroids. Bacteroids invading a nodule are no longer free-growing cells, instead depending on the plant cell for all resources. To elucidate the molecular mechanism of the bacteroid differentiation of Bradyrhizobium japonicum, a time-course analysis of bacteroid protein profiles was studied by 2-D gel electrophoresis in soybean nodules. Using proteomic analysis, protein expressions in soybean nodule bacteroids 7, 10, 14, 28, and 49 days after inoculation (DAI) were monitored. The time points coincide with the early stage of nodule formation (7–10 DAI), the onset of nitrogen fixation (14–28 DAI), and nodule senescence (49 DAI). In this study, 275 annotated protein spots were successfully identified, and a cluster analysis of their expression was performed. A large portion of putative, upregulated proteins were obse...
Databáze: OpenAIRE
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