Markers of melanoma progression in the blood serum structure

Autor: E. P. Lysenko, Olga N. Shatokhina, Viktoria Yu. Przhedetskaya, Galina V. Zhukova, Alla I. Shikhlyarova, Anastasia Ivanovna Zhadobina, Amira A. Akhmedova, Anna S. Goncharova, Viktoria V. Pozdnyakova, Olga V. Khokhlova, Yury Valentinovich Przhedetskiy, Oleg I. Kit
Rok vydání: 2019
Předmět:
Zdroj: Journal of Clinical Oncology. 37:e21035-e21035
ISSN: 1527-7755
0732-183X
Popis: e21035 Background: Lack of prognostic markers of melanoma to assess its progression, metastasis (MTS), and treatment effectiveness, determines the feasibility of studying biophysical aspects of the transmission of events from cellular to extracellular environment. Receipt and circulation of pathological proteins of melanoma (M) in the blood carries important information, and its trap is the phase transition of biofluids into a solid state. The purpose of the study was to determine markers of melanoma at the supramolecular level in solid-state samples of the blood serum (BS). Methods: 240 samples of BS obtained prior to the surgery from 60 patients with M were studied. The preparations were prepared by the Shatokhina-Shabalin method by creating a closed "analytic cell": dehydration of a BS drop (10 mcL) under a cover glass at 20–240С, humidity 65–70%, for 72 hours. The folding of M proteins was assessed by a polarized light microscopy (Leica DM LS2 microscope, x400-640) using photo and video control and the Morphotest program. Results: The initial stages of M development were characterized by the early appearance of single combined melanoform crystals — anisotropic microspherolites (Mi) embedded in the center of a large anisomorphone (lAM). As M progressed, Mi aggregation with basic lAMs was enhanced. Anisotropy of Mi increased, revealing clear sectors filled with melanin. In the later stages of M growth, melanoform aggregations dominated with point, filamentous, and chained melanin structures. The manifestation of MTS was accompanied by the destruction of the aggregations and the filling of the analytic cell space with numerous Mi. Conclusions: Correspondence of crystallogenesis of M melanomorphic markers in BS in tumor progression allows using the studied supramolecular factors to predict the development of melanoma.
Databáze: OpenAIRE
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