Abstract 382: Profiling angiogenic factors, RTK phosphorylation and inhibition events in breast cancer and endothelial cells using antibody arrays
| Autor: | Greta Wegner, Amy James, David J. Finkel |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
medicine.medical_specialty biology Angiogenin business.industry Angiogenesis Tyrosine phosphorylation medicine.disease Receptor tyrosine kinase Axitinib chemistry.chemical_compound Endocrinology Breast cancer Oncology chemistry Internal medicine medicine biology.protein Cancer research Phosphorylation business Tyrosine kinase medicine.drug |
| Zdroj: | Cancer Research. 73:382-382 |
| ISSN: | 1538-7445 0008-5472 |
| DOI: | 10.1158/1538-7445.am2013-382 |
| Popis: | Tumor growth and progression in breast cancer is dependent on angiogenesis, the growth of new vasculature from pre-existing vessels. Since invasive and in situ breast cancers express many angiogenic factors, we examined the profiles of angiogenesis-related proteins in the conditioned media from multiple breast cancer cell lines and their effect on activating receptor tyrosine kinases (RTKs) in Human Umbilical Vein Endothelial Cells (HUVEC). Antibody arrays were used to measure the secretion of multiple angiogenic factors including VEGF, TIMP-1, IGFBP-2, IGFBP-3, THSP-1, Angiogenin, and CXCL16. We then tested the capacity of these breast cancer cell line supernatants to induce receptor tyrosine kinase (RTK) phosphorylation in endothelial cells. After treatment with conditioned media, an increase in the tyrosine phosphorylation of VEGF R1 and VEGF R2 was observed using both antibody arrays and ELISA. Select small molecule VEG FR inhibitors were then screened for their effect on tyrosine phosphorylation events. Axitinib, Ki8751, and Sunitib effectively reduced VEGFR phosphorylation induced in endothelial cells by angiogenic factors released from these breast cancer lines. Citation Format: David J. Finkel, Amy E. James, Greta J. Wegner. Profiling angiogenic factors, RTK phosphorylation and inhibition events in breast cancer and endothelial cells using antibody arrays. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 382. doi:10.1158/1538-7445.AM2013-382 |
| Databáze: | OpenAIRE |
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