Dextran structural factors which affect binding to the lectin of Streptococcus cricetus
| Autor: | S Singh |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Polymers and Plastics biology Chemistry Streptococcus cricetus Streptococcus Organic Chemistry Lectin biology.organism_classification medicine.disease_cause Polysaccharide Streptococcaceae chemistry.chemical_compound Dextran Biochemistry Leuconostoc mesenteroides Materials Chemistry biology.protein medicine Bacteria |
| Zdroj: | Carbohydrate Polymers. 31:135-140 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/s0144-8617(96)00061-6 |
| Popis: | Oral bacteria, such as Streptococcus cricetus and S. sobrinus, are aggregated by high molecular weight fractions of the linear α-(1→6) dextran produced by Leuconostoc mesenteroides. The linkage specificity of this interaction with a cell surface lectin of the streptococci is high. As a further probe of factors affecting recognition, we prepared partially methylated samples of dextran of average MW of 2 × 106 and 104. 13C NMR spectroscopy proved to be a convenient method for determining both site and degree of monomethylation and dimethylation of the polymer: relative reactivity of the OH groups was established as 02: 04: 03 = 4.3: 1.7: 1.0. A methylation DS of only 0.17 caused almost complete loss of recognition by the lectin. A structural feature of dextran oligomers is suggested to explain this result. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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