Wound Healing Capacity, Antibacterial Activity, and GC-MS Analysis of Bienertia sinuspersici Leaves Extract
| Autor: | Faris J. M. Al-Imarah, Tarik Shwaish, Fatimah A Jasim |
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| Rok vydání: | 2019 |
| Předmět: |
History
Gram-negative bacteria Antioxidant biology Traditional medicine Chemistry ved/biology medicine.medical_treatment ved/biology.organism_classification_rank.species Bienertia sinuspersici biology.organism_classification Ascorbic acid Computer Science Applications Education Phytochemical medicine Agar diffusion test Wound healing Antibacterial activity |
| Zdroj: | Journal of Physics: Conference Series. 1294:062056 |
| ISSN: | 1742-6596 1742-6588 |
| Popis: | This study evaluates the wound healing efficacy of Bienertia sinuspersici leaves, a recently discovered species in the Arabian Gulf. A topical cream was formalized from the leaves and its wound healing activity was evaluated for incision, excision, and burn wound models. Bienertia sinuspersici extract was prepared and used for preliminary phytochemical screening, Gas Chromatography-MS analysis, antibacterial assay (disk diffusion method S. aureus, S. pyogenes, E. coli, and P. aeruginosa), antioxidant study, and to formulate a cream that was used for wound healing clinical trial. The presence of glycosides, saponins, and tannins is not confirmed; GC-MS analysis indicates the presence of 3-O-Methyl-d-glucose (a sugar, 44.29% concentration) and l-(+)-Ascorbic acid 2,6-dihexadecanoate (fat-soluble Ascorbic acid, 9.19% concentration). Average zone of inhibition for gram positive and gram negative bacteria were respectively 12-18 and 14-21 mm. Epithelization period for incisions wounds was 6-8 days (negative control: 12 days) with high keratin production and decreased scarring. Healing in excision wounds was steadier, faster and infection-less, contrary to controls. Bienertia sinuspersici leaves promote rapid and steady wound healing for both excision and incision wounds, also its antibacterial activity seems to induce a rapid, infection-less healing process. |
| Databáze: | OpenAIRE |
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