Cadexomer iodine provides superior efficacy against bacterial wound biofilms in vitro and in vivo
| Autor: | Jillian McMillan, Lei Shi, Shannon P. Tetens, David N. Earnest, Paul J. Renick, Eric Roche, Emma C. Forrest, Brett M. Kiedaisch, Daniel James Fitzgerald |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pseudomonas aeruginosa 030106 microbiology Biofilm Mupirocin Dermatology biochemical phenomena metabolism and nutrition Biology medicine.disease_cause Antimicrobial biology.organism_classification Microbiology 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry In vivo Staphylococcus aureus medicine Surgery Ex vivo Bacteria |
| Zdroj: | Wound Repair and Regeneration. 25:13-24 |
| ISSN: | 1067-1927 |
| DOI: | 10.1111/wrr.12497 |
| Popis: | Examination of clinical samples indicates bacterial biofilms are present in the majority of chronic wounds, and substantial evidence suggests biofilms contribute significantly to delayed healing. Bacteria in biofilms are highly tolerant of antimicrobials, and little data exist to guide the choice of anti-biofilm wound therapy. Cadexomer Iodine (CI) was recently reported to have superior efficacy compared to diverse wound dressings against Pseudomonas aeruginosa biofilms in an ex vivo model. In the current study, the strong performance of CI versus P. aeruginosa biofilm was confirmed using colony and colony drip-flow in vitro wound biofilm models. Similar in vitro efficacy of CI was also demonstrated against mature Staphylococcus aureus biofilms using the same models. Additionally, the rapid kill of mature S. aureus and P. aeruginosa colony biofilms was visualized by confocal microscopy using Live/Dead fluorescent stains. Superior in vitro efficacy of CI versus staphylococcal biofilms was further demonstrated against MRSA using multiple biofilm models with log reduction, Live/Dead, and metabolic endpoints. Comparator antimicrobial dressings, including silver-based dressings used throughout and other active agents used in individual models, elucidated only limited effects against the mature biofilms. Given the promising in vitro activity, CI was tested in an established mouse model of MRSA wound biofilm. CI had significantly greater impact on MRSA biofilm in mouse wounds than silver dressings or mupirocin based on Gram-stained histology sections and quantitative microbiology from biopsy samples (>4 log reduction in CFU/g versus 0.7-1.6, p |
| Databáze: | OpenAIRE |
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