Uric acid detection using uv-vis spectrometer
| Autor: | Hadi Manap, M Mohamad, Z R Abdul Rasad, N Norazmi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Spectrometer 010401 analytical chemistry Analytical chemistry Beer–Lambert law 02 engineering and technology 021001 nanoscience & nanotechnology medicine.disease_cause 01 natural sciences 0104 chemical sciences Absorbance chemistry.chemical_compound Wavelength symbols.namesake Ultraviolet visible spectroscopy chemistry medicine symbols Uric acid 0210 nano-technology Absorption (electromagnetic radiation) Ultraviolet |
| Zdroj: | IOP Conference Series: Materials Science and Engineering. 257:012031 |
| ISSN: | 1757-899X 1757-8981 |
| DOI: | 10.1088/1757-899x/257/1/012031 |
| Popis: | The aim of this research is to detect uric acid (UA) concentration using Ultraviolet-Visible (UV-Vis) spectrometer in the Ultraviolet (UV) region. Absorption technique was proposed to detect different uric acid concentrations and its UV absorption wavelength. Current practices commonly take a lot of times or require complicated structures for the detection process. By this proposed spectroscopic technique, every concentration can be detected and interpreted into an absorbance value at a constant wavelength peak in the UV region. This is due to the chemical characteristics belong to the uric acid since it has a particular absorption cross-section, σ which can be calculated using Beer's Lambert law formula. The detection performance was displayed using Spectrasuite sofware. It showed fast time response about 3 seconds. The experiment proved that the concentrations of uric acid were successfully detected using UV-Vis spectrometer at a constant absorption UV wavelength, 294.46 nm in a low time response. Even by an artificial sample of uric acid, it successfully displayed a close value as the ones reported with the use of the medical sample. It is applicable in the medical field and can be implemented in the future for earlier detection of abnormal concentration of uric acid. |
| Databáze: | OpenAIRE |
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