Enantioselective enzymatic synthesis of the α-hydroxy ketone (R)-acetoin from meso-2,3-butanediol
| Autor: | Alexander Scholz, Svenja Kochius, Hedda Merkens, Marion B. Ansorge-Schumacher, Jens Schrader, Andreas Vogel, Dirk Holtmann, Melanie Paetzold, Frank Hollmann |
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| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Ketone biology Process Chemistry and Technology Acetoin Enantioselective synthesis Bioengineering Nicotinamide adenine dinucleotide Biochemistry Chemical synthesis Catalysis Cofactor chemistry.chemical_compound chemistry biology.protein 2 3-Butanediol Organic chemistry Alcohol dehydrogenase |
| Zdroj: | Journal of Molecular Catalysis B: Enzymatic. 103:61-66 |
| ISSN: | 1381-1177 |
| DOI: | 10.1016/j.molcatb.2013.08.016 |
| Popis: | Acetoin (3-hydroxy-2-butanone) is an important flavour compound and is applied in cosmetics, pharmacy and chemical synthesis. In contrast to chemical syntheses or fermentations an enzymatic route facilitates enantioselective acetoin production. The discovery of a (S)-selective alcohol dehydrogenase enables a novel production process of (R)-acetoin from meso-2,3-butanediol. It was shown that the regeneration of oxidised nicotinamide adenine dinucleotide is a key point in preparative application of dehydrogenases for the oxidative route. An electrochemical regeneration system was successful combined with the ADH catalysed reaction. Up to 48 mM (R)-acetoin was produced in the reaction system while productivities up to 2 mM h−1 were reached. The possibility to apply an electrochemical system in a semi-preparative synthesis will stimulate further research of electroenzymatic processes with oxidoreductases. |
| Databáze: | OpenAIRE |
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