Evaluation of Bacterial Community Structure and Its Influence on Sulfide Oxidation in a Bio-Leaching Environment
| Autor: | Trevor C. Charles, Mazda Biglari, Ronald V. Nicholson, Jeno M. Scharer |
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| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Sulfide biology Chemistry Inorganic chemistry chemistry.chemical_element Acidithiobacillus engineering.material biology.organism_classification Microbiology Sulfur Iron bacteria Specific surface area Earth and Planetary Sciences (miscellaneous) engineering Environmental Chemistry Leaching (metallurgy) Pyrite Bacteria General Environmental Science Nuclear chemistry |
| Zdroj: | Geomicrobiology Journal. 26:44-54 |
| ISSN: | 1521-0529 0149-0451 |
| DOI: | 10.1080/01490450802602716 |
| Popis: | The mechanism of sulfide oxidation by adhering bacteria (direct oxidation mechanism) and by ferric ion in the aqueous phase was studied by quantitative assessment of bacterial activity on the sulfide surface. To probe for the principal bacterial species on the surface and in the supernatant, a library of DNA genes encoding portions of bacterial 16S rRNA was constructed. The PCR-amplified DNA from the bacterial populations was cloned employing PROMEGA's pGEM-T Easy Vector system. The clone frequency indicated that iron-oxidizing bacteria were dominant in the liquid phase, while Acidithiobacillus ferroixdans, which is both sulfur and iron oxidizer was the most prevalent on the sulfide surface. Samples of crystalline pyrite were exposed to the bacterial consortium to evaluate surface alterations caused by bacteria. Chemical (abiotic) oxidation experiments with ferric ion as the oxidant were carried out in parallel with the biological oxidation tests. Changes in the surface topography were monitored by atomic... |
| Databáze: | OpenAIRE |
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