Preparation of Protein with AviTag™ for Biotin-Based Capture

Autor:	Rashmi Rekha Devi, Dinesh Thiagaraj, Saravanan Kandan
Rok vydání:	2021
Předmět:	chemistry.chemical_classification Analyte chemistry.chemical_compound chemistry biology Biotin Biomolecule Biotinylation education Biophysics biology.protein Avidin
Zdroj:	Methods for Fragments Screening Using Surface Plasmon Resonance ISBN: 9789811615351
Popis:	Avidin tagged proteins take advantage where amine coupling fails to provide required interactions between protein and analyte. Immobilization of biomolecules by avidin–biotin-based methods is very common due to the robustness of the interaction. Preparation of AviTag™ protein for SPR is the primary work to initiate the binding studies. This chapter will outline the preparation of AviTag™ protein.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f7307ebeb20097d338555cf91edefc93 https://doi.org/10.1007/978-981-16-1536-8_4 Zobrazit plný text záznamu