Impact of Stevia rebaudiana Culturing in Liquid Medium: Elevation of Yield and Biomass, Mitigation of Steviol Glycosides

Autor:	Rabia Javed, Buhara Yücesan
Rok vydání:	2022
Předmět:	General Agricultural and Biological Sciences General Biochemistry Genetics and Molecular Biology General Environmental Science
Zdroj:	Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences. 59:69-75
ISSN:	2518-427X 2518-4261
Popis:	An efficient micropropagation system is developed by culturing nodal segments containing the axillary shoot buds on liquid Murashige and Skoog (MS) medium. Direct shoot and root formation are highly effective in both solid and liquid MS media without any plant growth regulators (PGRs). Interestingly, a significant difference in yield is obtained between solid and liquid cultures. It is revealed that a relatively higher amount of plant biomass is obtained after culturing for 4 weeks in a liquid MS medium. However, the shoots produced on solid MS medium produce a remarkable decline in all physiological parameters. On contrary, the bioactive steviol glycosides (rebaudioside-A and stevioside) content is higher in shoots grown in solid MS on a comparative basis, which could be compensated by higher yield. After hardening off, all the regenerants are effectively grown in the field with a negligible loss (
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f7303222d7149f69be1b05ed976199a8 https://doi.org/10.53560/ppasb(59-1)704 Zobrazit plný text záznamu