Resveratrol treatment attenuates the wound-induced inflammation in zebrafish larvae through the suppression of myeloperoxidase expression
| Autor: | Y.-F. Liao, J.-N. Tsai, Yau-Hung Chen, C.-C. Wen, M.-C. Chiou, Y.-H. Wang, Chien-Chung Cheng |
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| Rok vydání: | 2020 |
| Předmět: |
030309 nutrition & dietetics
Inflammation Resveratrol 01 natural sciences Green fluorescent protein 03 medical and health sciences chemistry.chemical_compound In vivo medicine Zebrafish Pharmacology chemistry.chemical_classification 0303 health sciences biology Phytoalexin fungi 010401 analytical chemistry food and beverages biology.organism_classification Molecular biology 0104 chemical sciences chemistry Myeloperoxidase biology.protein medicine.symptom Immunostaining Food Science |
| Zdroj: | Journal of Food and Drug Analysis. 19 |
| ISSN: | 2224-6614 |
| Popis: | Resveratrol, a polyphenolie phytoalexin found in many plants, was reported to exhibit anti-inflammatory effects, but its molecular mechanism is not fully understood. This study was aimed to investigate the anti-inflammatory effects of resveratrol in vivo using a zebrafish model of wound-induced inflammation. Caudal fin-wounded zebrafish larvae were treated with resveratrol for 8 h. Neutrophil recruitment was visualized in transgenic line ”Tg (mpx: GFP)” expressing GFP-tagged neutrophil-specific myeloperoxidase (mpx). The enzymatic activity of Mpx was evaluated by histochemical staining. Relative mRNA levels of mpx and eyclooxegenase-2 (cox2) were quantified by qRT-PCR, and the protein expression levels of Mpx and Cox2 were detected by immunostaining. Results showed that wound-induced neutrophil recruitment in zebrafish was nut affected by resveratrol, but Mpx enzymatic activity in zebrafish was significantly suppressed by resveratrol in a dote-dependent manner. Moreover, both mRNA and protein expression levels of Mpx and Cox2 were significantly down-regulated by resveratrol. Taken together, our results provide in viva evidence that the anti-inflammatory effects of resveratrol on wound-induced inflammation are significantly mediated through the suppression of Mpx and Cox2 at both transcriptional and protein levels, rather than the down-regulation of neutrophil recruitment. In conclusion, this in vivo zebrafish model can be readily applied to screen other potential anti-inflammatory compounds at a whole-organism level. |
| Databáze: | OpenAIRE |
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