Analytical application of immobilized acid urease for urea in flow streams
| Autor: | Kunio Matsumoto, Ikuo Satoh, Masashi Akahane |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
Chromatography Immobilized enzyme Urease biology Chemistry Calibration curve Metals and Alloys Polymer Porous glass Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound Enzymatic hydrolysis Materials Chemistry biology.protein Urea Electrical and Electronic Engineering Instrumentation Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 5:241-243 |
| ISSN: | 0925-4005 |
| Popis: | Acid urease is immobilized onto porous glass beads with controlled pore size and packed into a small polymer column. The simple biosensing system for determination of urea comprises the immobilized enzyme column and a flow-through microcalorimetric device (enzyme-thermistor). The heat produced in the enzymatic hydrolysis of urea is calorimetrically monitored with the device. The calibration graph is linear from 0.05 to 2.0 mM urea for 1.0 ml samples. The assay takes 6 min or less. The relative standard deviation for 1.0 mM urea is 1% (n = 10). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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