TRPA1 receptor is upregulated in human oral lichen planus
| Autor: | József Kun, Tamas Tornoczki, Anikó Perkecz, Lorenz Knie, Erika Pintér, Ágnes Bán, György Sétáló |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
food and beverages Biology medicine.disease Angiotensin II Proinflammatory cytokine stomatognathic diseases 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Immune system stomatognathic system Otorhinolaryngology Downregulation and upregulation Interferon Immunology medicine Oral lichen planus Oral mucosa Receptor General Dentistry psychological phenomena and processes medicine.drug |
| Zdroj: | Oral Diseases. 23:189-198 |
| ISSN: | 1354-523X |
| Popis: | Objective Oral lichen planus (OLP) is a chronic inflammatory disease of unknown etiology with antigen-specific and non-specific mechanisms. Transient Receptor Potential Ankyrin 1 (TRPA1) is a non-selective cation channel activated by noxious stimuli such as oxidative stress products evoking pain and release of proinflammatory mediators from sensory nerve endings culminating in neurogenic inflammation. Extraneuronal TRPA1s e.g. on immune cells possess yet unkown functions. Subjects and Methods We studied the buccal mRNA expression (qPCR) and protein localization (immunohistochemistry) of TRPA1 receptors, and key OLP mediator transcripts in oral mucosa samples of healthy volunteers (n=9), OLP patients (n=43) and OLP-like hyperkeratotic diseases (n=12). Results We measured 27.7 and 25.5-fold TRPA1 mRNA increase in OLP and OLP-like hyperkeratotic patients compared to healthy controls. TRPA1 transcripts elevated 2.4-fold in hypertensive OLP but not in hyperkeratotic patients compared to counterparts, reduced by 1.6-fold by angitensin-convertase inhibitor intake. TRPA1 messenger RNA was more coexpressed with transcripts of tumor necrosis factor α than with interferon γ. Keratinocytes, macrophages but not T-cells expressed TRPA1. Conclusions We provided evidence for the extraneuronal presence and upregulation of the proinflammatory TRPA1 receptor in buccal samples of patients with OLP. This may implicate the ion channel in the pathomechanism of OLP. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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