Determination of Cyanogenic Glycosides in Flaxseed by Barbituric Acid−Pyridine, Pyridine−Pyrazolone, and High-Performance Liquid Chromatography Methods

Autor:	B. D. Oomah, Giuseppe Mazza, M. Kobaisy
Rok vydání:	1996
Předmět:	chemistry.chemical_classification Chromatography Barbituric acid Cyanide Substrate (chemistry) Glycoside General Chemistry Linamarin High-performance liquid chromatography chemistry.chemical_compound Hydrolysis chemistry Cyanogenic Glucoside General Agricultural and Biological Sciences
Zdroj:	Journal of Agricultural and Food Chemistry. 44:3178-3181
ISSN:	1520-5118 0021-8561
DOI:	10.1021/jf950838j
Popis:	A crude enzyme extracted from flaxseed by acetone precipitation was used for the hydrolysis of cyanogenic glycosides to determine total cyanide in flaxseed and flaxseed-derived products. The hydrolysis of cyanogenic glycosides (linamarin, linustatin, and neolinustatin) as well as the endogenous substrate was dependent on the crude enzyme concentration and followed a first-order relationship. Two colorimetric methods using the crude enzyme extract and an established high-performance liquid chromatographic (HPLC) method were compared using 28 flaxseed samples. Total HCN values obtained by all three methods were not statistically different, although those obtained by the HPLC method were higher than those from the colorimetric methods. Keywords: Flaxseed; cyanogenic glycosides; method comparison; crude enzyme extract; Linum usitatissimum; barbituric acid−pyridine; HPLC
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f6e355e9847b28e7270eb4d4011fc9b7 https://doi.org/10.1021/jf950838j Zobrazit plný text záznamu