Molecularly Imprinted Polymer Solid-Phase Extraction (MISPE) for the Determination of Dinitro-ortho-cresol (DNOC) and Its Risk Assessment in Tomatoes
| Autor: | Novi Fitria, Sri Priatni, Bimastyaji Surya Ramadan, Muhammad Bachri Amran, Raden Tina Rosmalina |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Molekul. 18:29 |
| ISSN: | 2503-0310 1907-9761 |
| Popis: | In this study, the molecularly imprinted polymer solid-phase extraction (MISPE) method for the determination of dinitro-ortho-cresol (DNOC) before HPLC (High-performance liquid chromatography) analysis was conducted. This study aims to analyze the sorption behavior and the potential use of molecularly imprinted polymers (MIP) for DNOC extraction. MIP was prepared using a combination of methacrylic acid as the functional monomer; ethylene glycol dimethacrylate as the crosslinker, benzoyl peroxide as the initiator, and acetonitrile as the porogen. The results showed that the optimum adsorption of the material was achieved at pH 5 for a contact time of 30 min with an adsorption capacity of 137 mg/g. The ideal eluent for desorption was a mixture of methanol and acetic acid with a ratio of 3:1. The calculations showed that the adsorption process followed the Sips adsorption isotherm model (n = 0.967), which indicated adsorption on both homogeneous and monolayer surfaces. Reusability studies that were conducted via three consecutive adsorption-desorption cycles resulted in recovery values of 109.4%, 108.8%, and 101.1%. The concentration of DNOC obtained from tomato samples was 0.65 mg/kg with a recovery rate of 87.17%; this was within the tolerable range between 80% and 110%. Based on the high recovery and low LoQ, this method can be used to quantify DNOC quickly. |
| Databáze: | OpenAIRE |
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