| Autor: |
P. Krákora, P. Helbich, J. Jakoubková, J. Bubeník, M. Barešová, V. Viklický, V. Malasková |
| Rok vydání: |
1971 |
| Předmět: |
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| Zdroj: |
International Journal of Cancer. 8:503-513 |
| ISSN: |
0020-7136 |
| DOI: |
10.1002/ijc.2910080319 |
| Popis: |
A microassay in disposable tissue-culture plates was used to demonstrate a cell-mediated immune response against human renal adenocarcinomas. Leukocytes from 12 of the 19 patients with renal carcinoma were cytotoxic for autochthonous and allogeneic renal carcinoma cells in vitro. No reproducible cytotoxic effects were produced by leukocyte suspensions from patients with pulmonary or mammary carcinoma, or by leukocytes from healthy and non-neoplastic control subjects. No cytotoxicity was observed when cells of unrelated tumours, mesothelioma and urinary bladder carcinoma were used as target cells for leukocytes from a patient who reacted positively against autochthonous renal carcinoma cells. The results indicate that human renal adenocarcinomas possess tumour-associated antigens which cross-react with each other. Cytological and electron microscopic characteristics of cultivated renal carcinoma cells are described. Les auteurs ont procede a un microtest sur des lames de culture tissulaire a jeter apres usage afin de mettre en evidence la reponse immunitaire a mediation cellulaire envers les adenocarcinomes renaux humains. Les leucocytes de 12 malades sur les 19 qui portaient un carcinome renal etaient cytotoxiques a l'egard des cellules de carcinome renal allogeneiques et autochtones in vitro. Aucun effet cytotoxique reproductible n'a ete provoque par les suspensions de leucocytes de malades atteints de carcinomes pulmonaire ou mammaire ni par les leucocytes de temoins en bonne sante ou non cancereux. Aucune cytotoxicite n'a ete observee lorsque les cellules de tumeurs non apparentees (mesotheliome et carcinome vesical) ont ete utilisees comme cellules-cibles pour les leucocytes d'un malade qui reagissait positivement aux cellules autochtones de carcinome renal. Les resultats indiquent que les adenocarcinomes renaux humains possedent des antigenes associes a la tumeur qui ont entre eux des reactions croisees. Les auteurs decrivent la caracterisation cytologique ou par microscopie electronique des cultures de cellules de carcinome renal. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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