Lipid based drug delivery systems: Kinetics by SANS
| Autor: | T. N. Murugova, Daniela Uhríková, Oleksandr I. Ivankov, José A. Teixeira, Lukáš Hubčík, Alexander Búcsi, Tomáš Kondela |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030103 biophysics History Materials science Aqueous solution Kinetics Stacking Small-angle neutron scattering Computer Science Applications Education 03 medical and health sciences Crystallography 030104 developmental biology Pulmonary surfactant Drug delivery Lamellar structure Lipid bilayer |
| Zdroj: | Journal of Physics: Conference Series. 848:012007 |
| ISSN: | 1742-6596 1742-6588 |
| DOI: | 10.1088/1742-6596/848/1/012007 |
| Popis: | N,N-dimethyldodecylamine-N-oxide (C12NO) is a surfactant that may exist either in a neutral or protonated form depending on the pH of aqueous solutions. Using small angle X-ray diffraction (SAXD) we demonstrate structural responsivity of C12NO/dioleoylphospha-tidylethanolamine (DOPE)/DNA complexes designed as pH sensitive gene delivery vectors. Small angle neutron scattering (SANS) was employed to follow kinetics of C12NO protonization and DNA binding into C12NO/DOPE/DNA complexes in solution of 150 mM NaCl at acidic condition. SANS data analyzed using paracrystal lamellar model show the formation of complexes with stacking up to ~32 bilayers, spacing ~ 62 A, and lipid bilayer thickness ~37 A in 3 minutes after changing pH from 7 to 4. Subsequent structural reorganization of the complexes was observed along 90 minutes of SANS mesurements. |
| Databáze: | OpenAIRE |
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