| Autor: |
L E Bryan, Anthony B. Schryvers, Kurt Williams, Donald E. Woods, Tineke Schollaardt |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Serodiagnosis and Immunotherapy in Infectious Disease. 1:379-392 |
| ISSN: |
0888-0786 |
| DOI: |
10.1016/0888-0786(87)90049-7 |
| Popis: |
Oral administration of extracted lipopolysaccharide (LPS) from Pseudomonas aeruginosa was used to achieve serotype specific protection against P. aeruginosa in an ironloaded mouse model and a burned mouse model of infection. A significant delay to death could also be achieved using a neutropenic mouse model. In the iron-loaded mouse infection model protection could be achieved against three serotypes but not a fourth (serotype G). Protection against two serotypes could be achieved by simultaneous but not sequential feeding with both serotypes. Several oral immunization protocols were examined in the iron-loaded mouse model for efficacy and for toxicity. In protection studies, concentrations of LPS in the drinking water ranging from 2 to 200 μg/ml were used for periods from 1 to 40 days resulting in total intakes of 145 μg to 5·8 mg LPS. All protocols were successful in achieving protection provided that challenge occurred later than 2 days after the initial exposure to LPS. Toxicity studies were performed with similar amounts of LPS including an additional high dose (2 mg/ml LPS for 1 day, 14 mg total intake). No evidence of toxicity was observed for any dosage using a variety of criteria including pathological examination of tissue except for small temperature elevations in all but the lowest dose. Aerosol administration of LPS was also shown to provide protection against infection with the homologous strain. Passive transfer experiments demonstrated that protection was associated with specific antibody and not by cell mediated immunity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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