Procollagen Alpha 1 Type I: A Potential Aide in Histopathological Grading of Glioma

Autor: Niramol Savaraj, Alex J Furst, Howard J. Landy, Yingjie Wei, Lynn G. Feun, Chunjing Wu, Medhi Wangpaijit, Carlos Robles, M. Tien Kuo, Theodore J. Lampidis
Rok vydání: 2005
Předmět:
Zdroj: Cancer Investigation. 23:577-581
ISSN: 1532-4192
0735-7907
DOI: 10.1080/07357900500276915
Popis: Collagen type I production has been shown to play a role in malignant transformation. We examined procollagen type I expression in brain tumors and with histopathological grading. Expression levels of procollagen alpha 1 type 1 were determined in 5 glioma cell lines by RT-PCR, Northern, and Western blot analysis. In addition, 41 primary brain tumors and 2 metastatic lung cancers to the brain were examined by PCR. Of the 5 glioma cell line analyzed, 3 (glioma 1, SW-1783 and U-118) expressed procollagen alpha 1 type I and were sensitive to vitamin D3 (VD3). In contrast, 2 of the cell lines (U-373 and T-98G) lacked procollagen alpha 1 type 1 expression. In patients' samples, 14 of 15 anaplastic and low grade gliomas expressed procollagen alpha 1 type I, and 12 of the 14 expressed high levels. In contrast, only 12 of 21 high grade gliomas from patients expressed procollagen alpha 1 type1 and among these, only 4 of the 12 expressed high levels. Thus, there is an inversed correlation between procollagen alpha 1...
Databáze: OpenAIRE
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