Proposed reference method for iron in serum used to evaluate two automated iron methods

Autor:	A A Valcour, Mary Onoroski, George N. Bowers, Robert B. McComb, G A Krzymowski
Rok vydání:	1990
Předmět:	Detergent solubilization Blood Chemical Analysis Chromatography medicine.diagnostic_test Chemistry Biochemistry (medical) Clinical Biochemistry Analytical chemistry Disease control Biological fluid Colorimetry (chemical method) Serum iron medicine Hospital patients Reference standards
Zdroj:	Clinical Chemistry. 36:1789-1792
ISSN:	1530-8561 0009-9147
Popis:	The manual Reference Method of the Centers for Disease Control for serum iron (CDC/RM/Fe) and a semiautomated adaptation of it were used to evaluate two working methods: one, a detergent solubilization procedure for the Roche Cobas-Bio analyzer, the other, the Kodak Ektachem 700 procedure, based on dry-film technology. The CDC/RM/Fe and its semiautomated version gave essentially the same results for 40 sera from hospital patients. This semiautomated version was in turn compared with the two working procedures in a study involving 200 patients. Each of the working methods correlated well with the semiautomated CDC/RM/Fe method. Separate recovery and interference studies indicated satisfactory analytical recovery of iron in all cases, but the detergent solubilization method was found to be susceptible to interference by hemoglobin, lipemia, and bilirubin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f68462d1802b55c8d4481ce34d68c2e0 https://doi.org/10.1093/clinchem/36.10.1789 Zobrazit plný text záznamu