| Autor: |
Yeshwant Lal Avinash Jadhav, Rekha Raghunathan, William E. Haskins, Itay Raphael, Karan Kalsaria, Thomas G. Forsthuber, Darshit Shah, Anjali B. Purkar, Swetha Mahesula, Nazul H. Grimaldo, Manjushree Anjanappa, David Noriega, Carola Wenk, Michael Vaynberg, Venkat Kotagiri, Jonathan Gelfond, Vidya Pericherla |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
ELECTROPHORESIS. 33:3810-3819 |
| ISSN: |
0173-0835 |
| DOI: |
10.1002/elps.201200200 |
| Popis: |
We hypothesized that quantitative MS/MS-based proteomics at multiple time points, incorporating rapid microwave and magnetic (M(2) ) sample preparation, could enable relative protein expression to be correlated to disease progression in the experimental autoimmune encephalomyelitis (EAE) animal model of multiple sclerosis. To test our hypothesis, microwave-assisted reduction/alkylation/digestion of proteins from brain tissue lysates bound to C8 magnetic beads and microwave-assisted isobaric chemical labeling were performed of released peptides, in 90 s prior to unbiased proteomic analysis. Disease progression in EAE was assessed by scoring clinical EAE disease severity and confirmed by histopathologic evaluation for central nervous system inflammation. Decoding the expression of 283 top-ranked proteins (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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