EFFECT OF CHOLESTEROL DEPLETION ON CADHERINS EXPRESSION IN CELL LINE OF ORAL SQUAMOUS CELL CARCINOMA
| Autor: | Katiúcia Batista Silva Paiva, Rebeca Barros Nascimento, Elias Almeida Dos Santos, Tagna De Oliveira Brandão, Viviane Palmeira Da Silva, Elisa Kauark Fontes, Flávia Caló Aquino Xavier |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.diagnostic_test
business.industry Cadherin Mesenchymal stem cell Cell morphology Immunofluorescence Pathology and Forensic Medicine Blot stomatognathic diseases Cell culture Cytoplasm Cancer research Medicine Radiology Nuclear Medicine and imaging Dentistry (miscellaneous) Surgery Epithelial–mesenchymal transition Oral Surgery business |
| Zdroj: | Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. 129:e157-e158 |
| ISSN: | 2212-4403 |
| Popis: | The epithelial to mesenchymal transition (EMT) is the process, in which, the neoplastic cells lose epithelial phenotype, assuming a mesenchymal aspect. Among the proteins that contribute to EMT in oral squamous cell carcinoma (OSCC) there are E-cadherin and N-cadherin. Objective: To evaluate the protein expression of E-cadherin and N-cadherin after exposure to methyl-beta-cyclodextrin (MβCD) in OSCC cell line (SCC-9). Study Design: Protein expression was determined by Western blotting and immunofluorescence. Results: The cholesterol depletion resulted in the change of cell morphology, with the acquisition of mesenchymal phenotype. The protein expression of the cadherins in the treated SCC-9 was shown to be increased in relation to the parental sample. No difference was observed in E-cadherin and N-cadherin expression (P = .543 and P = .849 respectively, Kruskal-Wallis Test). Although the negative correlation was not statistically significant (P = .898; r = -0.08, Pearson), N-cadherin showed the highest median expression (median = 2557; SD = 0.261) in relation to E-cadherin (median = 4575; SD = 1.581). Furthermore, a decreased of E-cadherin membrane expression, and nuclear and cytoplasmic N-cadherin expression gain was observed on the immunofluorescence. Conclusions: The present study demonstrated that cholesterol depletion is able to regulate the protein expression of E-cadherin and N-cadherin, favoring EMT in OSCC. |
| Databáze: | OpenAIRE |
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