Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular Reconstruction and Engineering
| Autor: | Faleh Tamimi, Dongdong Fang, Li-Chieh Lin, Simon D. Tran, Mohamed-Nur Abdallah, Jack O. Jordan, Mohammed Bakkar, Michael Roskies |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Scaffold Chemistry Cellular differentiation Mesenchymal stem cell 02 engineering and technology Anatomy 021001 nanoscience & nanotechnology Chondrogenesis Cell biology Transplantation 03 medical and health sciences Paracrine signalling 030104 developmental biology medicine.anatomical_structure Tissue engineering medicine Bone marrow 0210 nano-technology |
| Zdroj: | Adult Stem Cells ISBN: 9781493967544 |
| DOI: | 10.1007/978-1-4939-6756-8_22 |
| Popis: | Multipotent mesenchymal stromal cells (MSC) derived from both the bone marrow and adipose tissue possess the ability to differentiate into multiple cell lineages, regulate the immune function by secreting numerous bioactive paracrine factors, and hold great potential in cell therapy and tissue engineering. When combined with three-dimensional (3D) scaffolds, MSC can be used for bone defect reconstruction and engineering. This protocol describes the isolation of bone marrow mesenchymal stromal cells (BMMSC) and adipose-tissue derived stem cells (ADSC) from rabbits for subsequent seeding on tissue-engineered 3D-printed scaffolds and transplantation into a rabbit-model with the goal of repairing large osseous mandibular defects (one quarter of the lower jaw is removed surgically). Steps to demonstrate the three cell differentiation lineage potentials of BMMSC and ADSC into osteocytes, adipocytes, and chondrocytes are described. A modified cell seeding method using syringes on scaffold is detailed. Creating a large mandibular bone defect, the rapid prototyping method to print a customized 3D-scaffold, the scaffold implantation procedure in rabbits, and microcomputed tomography (micro-CT) analysis are also described. |
| Databáze: | OpenAIRE |
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