Detection limit of the minor component monitoring engraftment after bone marrow transplantation

Autor:	M. Stenersen, D. Perckla, B.M. Dupuy
Rok vydání:	2008
Předmět:	Detection limit Bone marrow transplantation Immunology Genetics Biology DNA extraction Molecular biology Pathology and Forensic Medicine
Zdroj:	Forensic Science International: Genetics Supplement Series. 1:551-553
ISSN:	1875-1768
DOI:	10.1016/j.fsigss.2007.10.032
Popis:	In order to determine the detection limit of the minor component in recipient after bone marrow transplantations, analysis of different STR polymorphisms (ACTBP2, D11S554, D17S906, D7S1517, PentaE, D12S391) were performed. White blood cells from four voluntaries were isolated and visually counted by microscopy (Burcher) and diluted (100000cells/ml). Mixtures were made (% recipient: 0.6, 1.25, 1.5, 2.5, 5 and 25) prior to DNA extraction by chelex. The results show that the detection limit is between 0.5 and 1% for the minor component when using selected STRs for monitoring chimerisme.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f6504c8e2ceaebf05f1a33c28325401c https://doi.org/10.1016/j.fsigss.2007.10.032 Zobrazit plný text záznamu Full Text from ScienceDirect